| 货号 | 39219 |
| 克隆类别 | 164C819 |
| 别名 | FCC2, COCA2, HNPCC, hMLH1, HNPCC2 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 2 - 3 µg/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This MLH-1 antibody was raised against a synthetic peptide corresponding to human MLH-1. |
| 背景 | MLH-1(COCA2orHNPCC) heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in the presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. MLH-1(COCA2orHNPCC) introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore ensure that only the newly mutated DNA strand will be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH-1 is implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in the case of major DNA damages. MLH-1 heterodimerizes with MLH-3 to form MutL gamma which plays a role in meiosis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 83 kDa |
| 参考文献 |
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MLH-1 mAb tested by Western blot. The analysis was performed using Molt-4 whole-cell extract and MLH-1 mAb at a 2 µg/ml dilution. |
