货号 | 39627 |
克隆类别 | 3B9 |
同种亚型 | IgG1 |
反应种属 | Other (Wide Range) |
来源宿主 | Mouse |
应用 | Immunoprecipitation |
使用方法 | Validated Applications: IP: 2 - 5 µg per IP |
供应商 | Active Motif |
纯化方式 | Protein G Chromatography |
免疫原 | This HA Tag antibody was raised against a peptide containing multiple repeats of the HA tag epitope, YPYDVPDYA. |
背景 | HA Tag has been derived from the hemagglutinin protein of the human influenza virus for use as an epitope for the detection and purification of tagged recombinant proteins expressed in vivo. The HA tag is a small epitope and generally does not interfere with the normal function of recmobinant proteins. |
运输条件 | Blue Ice |
存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
计算分子量 | N/A |
参考文献 |
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HA Tag mAb tested by IP / Western blot. Whole-cell lysate (400 µg) from U2OS cells expressing an HA-tagged protein was immunoprecipitated with 2 µg of either control mouse IgG (lane 1) or HA Tag mAb (lane 2). Immunoprecipitates were run on an SDS-PAGE gel and analyzed by Western blot using a second HA tag antibody. |