| 货号 | 39541 |
| 克隆类别 | 3G4B20 |
| 别名 | NCL, C23 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:500 dilution WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Culture Supernatant |
| 免疫原 | This Nucleolin antibody was raised against a salt-extracted nuclear matrix preparation from MDCK cells. |
| 背景 | Nucleolin(NCL,C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Concentrated culture supernatant. |
| 计算分子量 | 100 kDa |
| 参考文献 |
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Nucleolin mAb (Clone 3G4B20) tested by immunofluorescence. Staining of HeLa cells with Nucleolin mAb (Clone 3G4B20) (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel). | |
Nucleolin mAb (Clone 3G4B20) tested by Western blot. HeLa whole-cell extract (15 µg/lane) probed with Nucleolin mAb (Clone 3G4B20) (1:2,000 dilution). |
