| 货号 | 39393 |
| 克隆类别 | 151 |
| 别名 | CLAP, mE10, CIPER, c-E10, CARMEN |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µg per IP WB: 0.5 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Bcl10 antibody was raised against full-length recombinant human Bcl10 protein. |
| 背景 | Bcl10 (B-cell CLL/lymphoma 10) protein promotes apoptosis and activation of NFκB (NFκB p50 and NFκB p65) via NIK and IKK (IKKα, IKKβ, IKKi/IKKε and IKKγ). Bcl10 protein contains a caspase recruitment domain (CARD) which interacts with other CARD domain containing proteins to regulate NFκB signaling. It is also a substrate for MALT1. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 33 kDa |
| 参考文献 |
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Bcl10 antibody (mAb) (Clone 151) tested by Immunoprecipitation. 10 µg of Bcl10 antibody was used to immunoprecipitate Bcl10 from 500 µg of K562 whole cell extract (lane 2). 10 µg of mouse IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Bcl10 antibody at a 1 µg/ml dilution. | |
Bcl10 antibody (mAb) (Clone 151) tested by Western blot. Bcl10 detection by Western blot. The analysis was performed using 20 µg HeLa nuclear extract and Bcl10 mAb (Clone 151) at a 1 µg/ml dilution. |
