| 货号 | 39279 |
| 克隆类别 | 4E12 |
| 同种亚型 | IgG1 |
| 反应种属 | Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 0.5 - 2 µg/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Myc Tag antibody was raised against peptide corresponding to amino acids 410-419 of human c-Myc (EQKLISEEDL). |
| 背景 | Myc Tag is a sequence derived from the human c-Myc protein that has been developed into an effective epitope for detection of tagged proteins expressed in vivo. Clone 4E12 exhibits superior detection of Myc-tagged proteins compared to the more common 9E10 clone. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 参考文献 |
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Myc Tag mAb tested by Western blot. Whole-cell extract (20 µg) of NIH/3T3 cells expressing a Myc-tagged PP2A-alpha subunit blotted with Myc Tag mAb (0.5 µg/ml). |
