| 货号 | 91143 |
| 别名 | KDM4A, JMJD2, JHDM3A, TDRD14A |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Enzyme-linked Immunosorbent Assay |
| 使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution Bead-based ELISA: 40 - 600 ng/ml Note: The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. Application Testing: Parental Monoclonal vs. Recombinant View PDF file |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A. |
| 背景 | AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® JMJD2A antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. JMJD2A(KDM4A,JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains. |
| 运输条件 | Blue Ice |
| 存放说明 | Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing. |
| 存储溶液 | Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. AbFlex™ recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, s |
| 计算分子量 | 130 kDa |
| 参考文献 |
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