| 货号 | 91135 |
| 别名 | HEED, WAIT1 |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | ChIP/ChIP-Seq/Enzyme-linked Immunosorbent Assay |
| 使用方法 | Validated Applications: ChIP-Seq: 4 µg per ChIP Bead-based ELISA: 4 - 26 ng/ml Application Testing: Parental Monoclonal vs. Recombinant View PDF file |
| 供应商 | Active Motif |
| 纯化方式 | Ni-NTA |
| 免疫原 | This EED antibody was raised against full-length recombinant human EED protein. |
| 背景 | AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® EED antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency.EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression. |
| 运输条件 | Blue Ice |
| 存放说明 | Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing. |
| 存储溶液 | Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. AbFlex™ recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, s |
| 计算分子量 | 57 kDa |
| 参考文献 |
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