| 货号 | 61664 |
| 克隆类别 | 2G1F9 |
| 同种亚型 | IgG2a |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rat |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 - 10 µg each ICC/IF: 1 - 2 µg/ml dilution WB: 0.5 - 2 µl/ml dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a peptide containing acetyl-lysine 9 of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys9 can also be mono-, di- or trimethylated. The methylation of this residue is often associated with transcriptional repression. However, acetylation of histone H3 Lys9 is associated with transcriptional activation of the genes. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 µg of chromatin from a human medulloblastoma cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify Histone H3K9ac binding sites. The image shows binding across a region of chromosome 22. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by immunofluorescence. Left: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Right: Hoechst. | |
Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by Western blot. HeLa nuclear extract (20 µg per lane) probed with Histone H3K9ac antibody (mAb) (1 µg/ml dilution). Lane 1: untreated cells. Lane 2: cells treated with sodium butyrate. |
