| 货号 | 61476 |
| 克隆类别 | 1B1-B2 |
| 同种亚型 | IgG3 |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 0.5 - 4 µg per ChIP WB: 0.1 - 1 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against a peptide within the C-terminal region of human histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 100mM sodium phosphate pH 8.0, 90mM trisodium citrate, 430mM sodium chloride. If crystals are noted, warm to room temperature and mix gently before use. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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H3 antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 µg of chromatin from both HeLa and K562 cells and 4 µg H3 antibody. ChIP DNA was used in qPCR with the gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
Histone H3 antibody (mAb) (Clone 1B1-B2) tested by Western blot. Histone H3 antibody detection by Western blot. The analysis was performed using 20 µg of HeLa nuclear extract and Histone H3 antibody at a 0.25 µg/ml dilution. |
