| 货号 | 61402 |
| 克隆类别 | NM11 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunoprecipitation/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 µg per ChIP ChIP-Seq: 5 µg each ICC/IF: 0.5 µg/ml dilution Published Applications: IP ICC/IF WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | The antibody was raised against full-length p300 purified from human 293 cells. |
| 背景 | p300 (E1A binding protein p300) functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as an acetyltransferase for nonhistone targets. Acetylates Lys-131 of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 300 kDa |
| 参考文献 |
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p300 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million LNCaP cells and 5 µl of p300 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify p300 binding sites. p300 along with H3K4me1 are markers of active enhancer elements and are therefore expected to co-localize. A sampling of the p300 ChIP-Seq data shows the expected co-localization of p300 and H3K4me1. | |
p300 antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million LNCaP cells and 4 µg of p300 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
p300 antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with p300 antibody at a 0.5 µg/ml dilution. |
