| 货号 | 61400 |
| 克隆类别 | 9B1-2G6 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 0.5 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against a synthetic branched peptide corresponding to amino acids surrounding Lys9 of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Methylation of lysines occur as four states: unmethylated (me0), monomethyl (me1), dimethyl (me2) and trimethyl (me3). The me0 state of lysine is recognized as biologically relevant and a number of proteins containing PhD fingers, ADD and WD40 domains are known to associate with unmodified lysines. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3K9me0 mAb tested by Western blot. Histone H3K9me0 detection by Western blot. The analysis was performed using HeLa acid extract. | |
Histone H3K9me0 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K9me0 mAb for detection of unmethylated Lys9 of Histone H3. 50 pmol of recombinant Histone H3 proteins containing various modifications were spotted onto PVDF and probed with the antibody at 1 µg/mL. Lane 1: Histone H3 unmodified. Lane 2: Histone H3K4me3. Lane 3: Histone H3K9me3. Lane 4: Histone H3 K9ac. Lane 5: Histone H3 K14ac. Lane 6: Histone H3K4ac. Lane 7: Histone H3 S10ph. |
