| 货号 | 39672 |
| 克隆类别 | 6F8-D9 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µg per ChIP WB: 1 - 2 µg/ml dilution Slight reactivity towards histone H4 dimethyl Lys20 might be observed under certain conditions. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Histone H4 trimethyl Lys20 antibody (mAb) was raised against a peptide containing trimethyl Lys20 of Histone H4. |
| 背景 | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 8 kDa |
| 参考文献 |
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Histone H4 trimethyl Lys20 mAb (Clone 6F8-D9) tested by ChIP. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 5 µg of Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for human SAT-alpha repeats. Data are presented as Fold Enrichment of the ChIP antibody signal compared to the negative control IgG (which has been normalized to 1.0) using the ddCT method. | |
Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) tested by Western blot. HeLa nuclear extract (20 µg per lane) probed with Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) (2 µg/ml dilution). | |
Histone H4 trimethyl Lys20 mAb (Clone 6F8-D9) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) for trimethyl-Lys20 of histone H4. Modified and unmodified peptides were spotted onto PVDF and probed with Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) at a dilution of 2 µg/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: monomethyl Lys20 peptide. Lane 2: dimethyl Lys20 peptide. Lane 3: trimethyl Lys20 peptide. Lane 4: Unmodified Lys20 peptide. |
