| 货号 | 61262 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP ChIP-Seq: 10 µl each WB: 1:500 - 1:1,000 dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This CTBP2 antibody was raised against a peptide in the C-terminal region of human CTBP2. This antibody recognizes both isoform 1 and 2. |
| 背景 | TheCTBP2 (C-terminal binding protein 2) gene encodes a protein that is a corepressor targeting diverse transcription regulators. It functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses. Isoform 2 is also phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 50 kDa |
| 参考文献 |
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CTBP2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse liver and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify CTBP2 binding sites. The image shows binding across a regions of chromosome 11. You can view the complete data set in the UCSC Genome Browser, starting here. | |
CTBP2 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (30 µg) probed with CTBP2 antibody (1:500). |
