| 货号 | 61258 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 10 µl per IP WB: 1:500 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This EDF1 antibody was raised against a peptide within the N-terminal region of human EDF1. |
| 背景 | TheEDF1 (endothelial differentiation-related factor 1, MBF1) gene encodes a protein that may regulate endothelial cell differentiation. This protein has been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. It also enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 18 kDa |
| 参考文献 |
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EDF1 antibody (pAb) tested by Immunoprecipitation. 10 µl of EDF1 antibody was used to immunoprecipitate EDF1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the EDF1 antibody at a dilution of 1:500. | |
EDF1 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (30 µg) probed with EDF1 antibody (1:500). |
