| 货号 | 61242 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This EZH2 phospho Thr345 antibody was raised against a peptide containing phosphothreonine 345 of mouse EZH2 (enhancer of zeste homolog 2 (Drosophila)) |
| 背景 | EZH2 – (Enhancer of Zeste homolog 2, also designated Enx1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. Threonine 345 of mouse Ezh2 is phosphorylated in a cell cycle-dependent manner and may play a role in the interaction of Ezh2 with HOTAIR and the 59-RepA repeats of Xist. (see reference) |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
EZH2 phospho Thr345 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of EZH2 phospho Thr345 antibody. Modified and unmodified peptide were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. Decreasing amounts of peptide were spotted in each row. Lane 1: Peptide phosphorylated at threonine 345. Lane 2: Unmodified EZH2 peptide. |
