| 货号 | 61056 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This BLM antibody was raised against a peptide within the N-terminal region of human BLM. |
| 背景 | BLM (Bloom Syndrome protein, RECQ2, RECQL3) is a DNA helicase similar to E. coli RecQ protein that is mutated in patients with Bloom Syndrome, an autosomal recessive disorder characterized by growth deficiency, sun sensitive skin, cancer predisposition and chromosomal instability. The five human RecQ helicases play critical roles in DNA recombination, replication and repair. BLM helps to resolve aberrant chromosome structures generated during DNA replication. It exists in a complex with topoisomerase IIIα, RMI1/BLAP75 and RMI2 and it is thought that BLM complexes are recruited to resolve Holliday junction intermediates. BLM is heavily phosphorylated, especially during mitosis. BLM has a role during meiosis as well, as BLM males are sterile and BLM females have reduced fertility. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 165 kDa |
| 参考文献 |
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Western blot of BLM antibody. Nuclear extract of HeLa cells (30 µg per lane) probed with BLM antibody (pAb) at a dilution of 1:500. |
