| 货号 | 39866 |
| 同种亚型 | Serum |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µl per ChIP WB: 1:1,000 - 1:5,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This SIN3A antibody was raised against a peptide derived from the N-terminus of human SIN3A. |
| 背景 | SIN3A(Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 155 kDa |
| 参考文献 |
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SIN3A antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from TM4 Sertoli cells and 5 µl of SIN3A antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
SIN3A antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 μg) probed with SIN3A antibody at a dilution of 1:5,000. |
