| 货号 | 39780 |
| 同种亚型 | Serum |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 5 - 10 µl per IP WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This TORC2 antibody was raised against a peptide at the C-terminus of human TORC2. |
| 背景 | TORC2(Transducer of regulated cAMP response element-binding protein 2) (CRTC2 or CREB-regulated transcription coactivator 2) is a coactivator of transcription through the CREB1 signaling pathway, independent of phosphorylation at serine 133. Involved in metabolic signaling pathways, it is responsive to glucagon signaling and regulates gluconeogenesis. It is a potent activator of mitochondrial biogenesis in muscle cells. TORC2 is inactivated by phosphorylation at serine 171 (in response to both insulin signaling and the activation of AMPK), which facilitates recognition by 14-3-3 protein and subsequent translocation to the cytoplasm. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 85 kDa |
| 参考文献 |
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TORC2 antibody (pAb) tested by Immunoprecipitation. 10 µl of TORC2 antibody was used to immunoprecipitate TORC2 from 400 µg of HEK293 whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the TORC2 antibody at a dilution of 1:2,000. | |
TORC2 antibody (pAb) tested by Western blot. Detection of TORC2 by Western blot analysis. Whole cell extract (20 µg) of NIH/3T3 (lane 1) and HEK293 (lane 2) blotted with TORC2 antibody at a dilution of 1:2,000. |
