| 货号 | 39744 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: ChIP: 2 - 10 µl per ChIP IP: 2 - 10 µl per IP WB: 1:500 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This PARP-2 antibody was raised against a recombinant protein corresponding to the C-terminal region of human PARP-2. |
| 背景 | PARP-2 encodes a chromatin-associated enzyme, a poly-ADP ribosyl transferase, which modifies various nuclear proteins by poly-ADP ribosylation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. PARP-2 is cleaved by caspase enzymes during the process of apoptosis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 66 kDa |
| 参考文献 |
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PARP-2 antibody tested by ChIP analysis. Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input. | |
PARP-2 antibody used for immunoprecipitation. Western blot of immunoprecipitation using the PARP-2 antibody. Lane 1: input (AC16 whole cell lystate). Lane 2: IP using pre-immune serum. Lane 3: IP using PARP-2 antibody. | |
PARP-2 antibody tested by Western blot. Lane 1: U2OS nuclear extract (20 µg). Lane 2: A549 whole-cell extract(20 µg). Lane 3: Hep G2 whole-cell extract (20 µg). Lane 4: Jurkat cell nuclear extract (20 µg). These were probed with PARP-2 antibody at a dilution of 1:500. |
