| 货号 | 39732 |
| 同种亚型 | Serum |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 5 µl per ChIP ChIP-Seq & ChIP-chip: 5 µl each WB: 1:500 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This SAP30 antibody (pAb) was raised against recombinant full-length human SAP30 protein. |
| 背景 | SAP30, also known as Sin3A-associated protein, 30kDa, is part of the SIN3 corepressor complex, that also includes SIN3, SAP18, HDAC1, HDAC2, RbAp46 and RbAp48. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 30 kDa |
| 参考文献 |
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SAP30 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from the K-562 human myelogenous leukemia cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify SAP30 binding sites. The image on the left shows SAP30 binding at transcriptional start sites across a 2.4 million bp region on chromosome 1. The image on the right is zoomed in to show SAP30 binding at two other start sites. | |
SAP30 antibody (pAb) tested by Western blot. Detection of SAP30 by Western blot analysis. Lane 1: Whole cell extract of A549 cells (20 µg). Lane 2: Nuclear extract of HeLa cells (20 µg). Both were probed with SAP30 antibody (pAb) at a 1:500 dilution. |
