STAT2 phospho Tyr689 antibody (pAb), sample【科瑞奥博】

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STAT2 phospho Tyr689 antibody (pAb), sample

货号： 39612 基本售价： 1435.5 元规格： 10 ul

产品信息

概述
货号	39612
同种亚型	IgG
反应种属	Human
来源宿主	Rabbit
应用	Western Blot/Dot Blot
使用方法	Validated Applications: WB: 1:500 - 1:1,000 dilution

性能
供应商	Active Motif
纯化方式	Affinity Purified
免疫原	This STAT2 phospho Tyr689 antibody was raised against a peptide containing phospho Tyr689 of human STAT2.
背景	STAT2(signal transducer and activator of transcription 2) is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT2 has been shown to be activated by IFNα and IFNβ, and to associate with the transcription factors p48 and p300.
运输条件	Blue Ice
存放说明	Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
存储溶液	Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
计算分子量	110 kDa
参考文献

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参考图片

STAT2 phospho Tyr689 pAb tested by Western blot.
HeLa whole-cell extract (20 µg per lane) was probed with STAT2 phospho Tyr689 pAb (1:500).
Lane 1: No treatment.
Lane 2: Cells treated with IFNα (2500 units/ml for 30 minutes).

STAT2 phospho Tyr689 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of STAT2 phospho Tyr689 pAb for STAT2 phospho Tyr689. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.