| 货号 | 39560 |
| 同种亚型 | Serum |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 8 µl per ChIP WB: 1:5,000 - 1:50,000 dilution Published Applications: ChIP ChIP-chip See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This PARP-1 N-terminal antibody was raised against a His-tagged fusion protein corresponding to the N-terminal half of human PARP-1 was used to generate this PARP-1 antibody. |
| 背景 | PARP-1 N-terminal(ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1(ADPRT) occurs following caspase activation during apoptosis. For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG |
| 计算分子量 | 120 kDa |
| 参考文献 |
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PARP-1 N-terminal antibody tested by ChIP analysis. Chromatin IP performed with chromatin from 3T3 cells using 8 µl of PARP-1 N-terminal antibody or rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PPARG2 gene. Data are presented as percent of input from cells with a PARP-1 knock-down (black bars) or a control knock-down (gray bars). | |
PARP-1 N-terminal antibody tested by Western blot. HeLa cell nuclear extract (20 µg per lane) was probed with PARP-1 N-terminal antibody at a dilution of 1:20,000. |
