| 货号 | 39552 |
| 同种亚型 | Serum |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This HMG-1 antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 167-182 of human HMG-1. |
| 背景 | High mobility group proteins (HMG) are non-histone chromatin-associated proteins involved in DNA organization or regulation of transcription. Constitutively expressed in the nucleus of eukaryotic cells, HMGs share functional motifs that bind specific DNA structures (minor grooves) and induce conformational changes without specificity for target sequences. A Class II HMG member, HMG-1 binds to bent or distorted DNA such as four-way DNA junctions, synthetic DNA cruciforms, kinked cisplatin-modified DNA, DNA bulges and cross-overs in supercoiled DNA. It can cause looping of linear DNA, thus functioning as an architectural element that facilitates interactions of neighboring proteins and DNA by creating favorable DNA conformations. Extremely versatile, HMG-1 and HMG-2 are involved in numerous DNA events in the nucleus such as DNA repair, replication, recombination and transcription. Its nuclear localization and affinity for DNA is regulated through phosphorylation and acetylation, and has been found to have a dynamic relationship with chromatin. HMG-1 has been shown to stimulate the binding of a variety of sequence-specific DNA-binding proteins such as p53 in vitro, playing an important role in DNA architecture and transcriptional regulation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 32 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
HMG-1 pAb tested by Western blot. Detection of trimethylated HMG-1 by Western blot. The analysis was performed using 20 µg HeLa acid extract and HMG-1 pAb at a 1:2,000 dilution. |
