| 货号 | 39398 |
| 同种亚型 | Serum |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:250 - 1:500 dilution WB: 1:1,000 - 1:5,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This NF-1A antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 478-492 of human NF-1A. |
| 背景 | NF-1A(NF-1,Nuclear factor-1) is a ubiquitous 47 kDa dimeric DNA-binding protein whose recognition sequence, TGG(C/A)N(5)GCCAA, is found in the genomes of a number of DNA viruses. The same sequence occurs frequently in the human genome. The NF-1 protein stimulates initiation of adenovirus DNA replication in vitro and is capable of stimulating the transcription of genes in cooperation with other factors, such as the estrogen receptor. The Nuclear factor-1 (NF-1) family of transcription factors are encoded by the genes NF-1A, NF-1B2, NF-1C and NF-1X and feature a highly conserved N-terminal DNA binding region and a variable C-terminal transcriptional activation or repression domain. NF-1 binding controls the expression of many target genes, including insulin, TGF-β, nucleotide, steroid hormone and vitamin synthetic genes, TNFα and FSH. NF-1 transcripts can be differentially spliced and the resultant protein variants homo- and heterodimerize, allowing for multiple potential transcriptional activation and repression activities. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 60 kDa |
| 参考文献 |
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NF-1A antibody (pAb) tested by immunofluorescence. Detection of NF-1A antibody by immunofluorescence. Human Astrocytes-cerebellar (HA-c) cells were stained with NF-1A antibody at a 1:500 dilution. Green: NF-1A antibody (pAb) Red: Vimentin |
