| 货号 | 39292 |
| 同种亚型 | Serum |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 dilution Published Applications: see references Ikaros may be present as multiple protein isoforms due to alternative splicing. Commonly, the two largest isoforms are detected as seen in the example Western blot image. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros. |
| 背景 | Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 62 kDa |
| 参考文献 |
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Ikaros pAb tested by Western blot. Detection of Ikaros by Western blot analysis. Ikaros was detected using a 1:1,000 dilution of Ikaros pAb with 20 µg mouse spleen tissue extract. |
