| 货号 | 39290 |
| 同种亚型 | Serum |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunofluorescence/Immunohistochemistry/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:500 - 1:2,000 dilution WB: 1:500 - 1:1,000 dilution Published Applications: ICC/IF IHC See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Mi-2B antibody was raised against a recombinant protein corresponding to amino acids 22-155 of mouse Mi-2 beta. |
| 背景 | Mi-2β(Mi-2beta,CHD4,chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP). |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 240 kDa |
| 参考文献 |
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Mi-2 beta antibody tested by immunofluorescence. Top left: HeLa cells stained with Mi-2 beta antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images. | |
Mi-2 beta antibody tested by Western blot. Whole-cell extract of mouse splenocytes stained with Mi-2 beta antibody (1:1,1000). |
