| 货号 | 39230 |
| 同种亚型 | Serum |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This JARID1C / KDM5C antibody was raised against a peptide corresponding the C-terminus of human JARID1C. |
| 背景 | Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of a family of histone demethylase enzymes that contain a Jumonji-N (Jmj-N) and Jumonji-C (Jmj-C) domain in addition to an AT-rich interacting domain (ARID). JARID1C also contains 2 PHD domains and a PLU-1 domain, and a putative DNA binding module. JARID1C demethylates histone H3 that has been di- or trimethylated at lysine 4, and functions as a repressor of transcription. JARID1C also participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Mutations in the JARID1C gene have been associated with X-linked mental retardation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 180 kDa |
| 参考文献 |
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.JARID1C / KDM5C pAb tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human MIA PaCa-2 pancreatic carcinoma cells and 10 µl JARID1C / KDM5C antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
JARID1C / KDM5C pAb tested by Western blot. Nuclear extract of HeLa cells (20 µg) probed with JARID1C / KDM5C pAb (1:1,000) |
