| 货号 | 39199 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 4 µl per ChIP WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This RbAp46/48 antibody was raised against recombinant human RbAp46 protein. |
| 背景 | RbAp46(Retinoblastoma protein associated protein 46) and RbAp48(Retinoblastoma protein associated protein 48) are highly homologous histone chaperones that play key roles in protein complexes that function to establish and maintain chromatin structure. RbAp46/48 are found in a number of protein complexes involved in chromatin remodeling (Mi-2β, NURF & NURD), transcriptional repression (Prc2, Sin3) and chromatin assembly (CAF1). Both RbAp46andRbAp48 are specifically found in the catalytic cores of histone deacetylase (HDAC) complexes that promote transcriptional repression of target genes, including those repressed by the retinoblastoma tumor suppressor protein. RbAp46 binds to and enhances the activity of the type B histone acetyltransferase HAT1, an enzyme that acetylates histone H4 specifically at its Lys5 and Lys12 residues prior to their incorporation into nucleosomes during replication. RbAp48 is an evolutionarily conserved subunit of the chromatin assembly factor-1 (CAF-1) complex, where it associates with two other subunits, known as p150 and p60 in human cells. It is also reported that E2F-1 and RbAp48 are physically associated in the presence of Rb and histone deacetylase, suggesting that RbAp48 could be involved in transcriptional repression of E2F-responsive genes. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 46 and 48 kDa |
| 参考文献 |
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RbAp46/48 antibody (pAb) tested by ChIP ChIP performed on MCF7 cell chromatin (40 μg) using 39198. PCR was performed using primers specific for the promoter region of the human SNAIL gene. Lane 1: Input DNA control. Lane 2: ChIP using 4 μl of 39198. Lane 3: Duplicate. Lane 4: Rabbit IgG as control (-). Lane 5: Duplicate. Lane 6: PCR mix. | |
RbAp46/48 pAb tested by Western blot. HeLa cell nuclear extract (10 µg per lane) was probed with RbAp46/48 pAb (1:500 dilution). |
