| 货号 | 39193 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Other (Wide Range), Rat |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 2 - 10 µl per ChIP WB: 1:250 - 1:2,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This PP2A antibody was raised against a peptide corresponding to amino acids 288-303 of human PP2A. |
| 背景 | PP2A–Protein Phosphatase 2A beta subunit is the catalytic subunit of protein phosphatase 2A. PP2A is involved in the regulation of a number of signaling pathways, and it is implicated in the negative control of cell growth and division. Mutations of PP2A occurs in many types of cancers. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Peptide affinity purified IgG containing 30% glycerol, 0.035% sodium azide and 1 mg/ml BSA. Sodium azide is highly toxic. |
| 计算分子量 | 36 kDa |
| 参考文献 |
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PP2A antibody (pAb) tested by ChIP analysis. Chromatin IP performed using Swiss 3T3 Chromatin (approximately 5 million cells treated with anisomycin and fixed at 0.5 and 3 hours) using 2.5 µl of PP2A antibody or the equivalent amount of IgG as a negative control. | |
PP2A pAb tested by Western blot. Nuclear extract of HeLa cells was probed with PP2A pAb (1:1,000 dilution). |
