| 货号 | 39191 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:1,000 - 1:2,500 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This MEP50 antibody was raised against full-length human MEP50 with an N-terminal HIS tag. |
| 背景 | MEP50 (also designated WDR77) is an important component of the methylosome, a large (20S) protein arginine methyltransferase complex that contains PRMT5. The methylosome functions to modify specific arginines to dimethylarginines in the arginine- and glycine-rich domains of several spliceosomal Sm proteins. This modification targets these proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein (snRNP) core particles. A 50 kDa WD repeat protein MEP50 binds to JBP1 and a subset of Sm proteins and is thought to provide an interface for multiple protein interactions between methylosome proteins. The ability of MEP50 to interact with multiple proteins suggests it is important for targeting the Sm as well as other yet unknown proteins to the methylosome for symmetrical dimethylation. MEP50 specifically interacts with CTD phosphatase FCP1 (CTDP1), a protein that may link the processes of transcriptional elongation and splicing, and with Suz12, a polycomb group protein involved in transcriptional repression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 37 kDa |
| 参考文献 |
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MEP50 pAb tested by Western blot. Whole-cell extract of HeLa cells (20 µg per lane) was probed with MEP50 pAb at a dilution of 1:2,500 dilution (lane 1). In lane 2, the antibody was pre-incubated with 1 µM recombinant MEP50 protein, eliminating the band and demonstrating the specificity of MEP50 pAb for MEP50. |
