| 货号 | 61710-AM |
| 克隆类别 | 35C1 |
| 同种亚型 | IgG2b |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunoprecipitation/Immunofluorescence/Flow Cytometry/Immunocytochemistry |
| 使用方法 | Validated Applications: WB: 1 - 2 µg/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. Published Applications: FACS,IF/ICC,IP,&WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against full-length recombinant human Aurora A protein. |
| 背景 | Aurora A (Aurora Kinase A) is a mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 50 kDa |
| 参考文献 |
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Aurora A antibody (mAb) (Clone 35C1) tested by Western blot. Aurora A antibody detection by Western blot. The analysis of Aurora A was performed using HeLa cytoplasmic cell extract (20µg) and Aurora A antibody at a 1 µg/ml dilution. |
