| 货号 | 61706 |
| 克隆类别 | 8E3 |
| 同种亚型 | IgG1 |
| 反应种属 | Mouse |
| 来源宿主 | Rat |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 2 - 10 µg/ml dilution WB: 0.5 - 2 µg/ml dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against a peptide within the N-terminal region of mouse DHX9. |
| 背景 | DHX9 (DEAH (Asp-Glu-Ala-His) Box Helicase 9) unwinds double-stranded DNA and RNA in a 3 to 5 direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Positively regulates HIV-1 LTR-directed gene expression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 150 kDa |
| 参考文献 |
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DHX9 antibody (mAb) (Clone 8E3) tested by Immunofluorescence. Left: Formaldehyde fixed L929 cells stained with DHX9 antibody (mAb). Right: Hoechst. | |
DHX9 antibody (mAb) (Clone 8E3) tested by Western blot. DHX9 antibody detection by Western blot. The analysis was performed using 30 µg of either NIH-3T3 nuclear extract (lane 1), NIH-3T3 cytoplasmic extract (lane 2), or HeLa nuclear extract (lane 3) and DHX9 antibody (mAb) at a 2 µg/ml dilution. |
