| 货号 | 61626 |
| 克隆类别 | 5B7 |
| 同种亚型 | IgG2a |
| 反应种属 | Human, Monkey, Mouse |
| 来源宿主 | Rat |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 2 - 10 µg/ml dilution WB: 0.5 - 2 µg/ml dilution Published Applications: ICC/IF See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a recombinant protein corresponding to amino acids 213-295 of human SMARCA4. |
| 背景 | SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4) is the central catalytic ATPase subunit of numerous chromatin-remodeling complexes, including SWI/SNF. These complexes are able to use energy from ATP hydrolysis to physically disrupt chromatin architecture of target promoters and facilitate the binding of transcription factors to nucleosomal DNA. SMARCA4 is a co-regulator of transcription and has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. This protein plays a major role in many cellular processes such as DNA replication, repair and recombination. Mammalian SMARCA4 is usually associated with approximately 10-12 BAF subunits or other proteins involved in regulation of gene expression. SMARCA4 is part of the stem cell-specific BAF complex, esBAF, and is involved in regulating stem cell pluripotency. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 180 kDa |
| 参考文献 |
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SMARCA4 antibody (mAb) (Clone 5B7) tested by immunofluorescence. Top: HeLa cells stained with SMARCA4 antibody (mAb). Bottom: Hoechst staining. | |
SMARCA4 antibody (mAb) (Clone 5B7) tested by Western blot. Whole cell extracts were probed with SMARCA4 antibody (mAb). Lane 1: L929 cells. Lane 2: HeLa cells. Lane 3: Cos cells |
