| 货号 | 61602 |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Enzyme-linked Immunosorbent Assay |
| 使用方法 | Validated Applications: Elisa: Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | Human TNF. |
| 背景 | TNF (Tumor Necrosis Factor) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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TNF antibody (mAb) tested by ELISA. Recombinant TNF protein or a negative control protein was conjugated to Luminex® microspheres and then incubated in the same well with a serial dilution of TNF antibody (mAb). Biotinylated anti-mouse IgG followed by Streptavidin-PE was used to detect the bound TNF antibody . Mean Fluorescence Intensity (MFI) for each protein was measured in a Luminex® 200™. |
