| 货号 | 61578 |
| 克隆类别 | 7A9-3A3 |
| 同种亚型 | IgG1k |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunoprecipitation/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: IP: 5 µg per IP ICC/IF: 0.5 - 2 µg/ml dilution WB: 0.1 - 1 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design. |
| 背景 | Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3-5 exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 160 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Cas9 antibody (mAb) tested by Immunoflourescence. HeLa cells were transiently transfected with flag-tagged Cas9 for 48 hours and then stained. Top: Cas9 antibody (mAb) at a 0.5 µg/ml dilution. Bottom: Stained with Hoechst. | |
Cas9 antibody (mAb) tested by Western blot. HEK293 cells were transiently transfected with myc-tagged Cas9 (lane 2) or untransfected (lane 1), lysates prepared 40 hours post transfection, and probed with Cas9 antibody (mAb) at a 0.5 µg/ml dilution. |
