| 货号 | 61506 |
| 克隆类别 | 1F1 |
| 同种亚型 | IgG2b |
| 反应种属 | Human |
| 来源宿主 | Rat |
| 应用 | ChIP/Immunofluorescence/Electrophoretic Mobility Shift Assay/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 0.5 µg/ml dilution Published Applications: ChIP EMSA See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a full-length recombinant protein corresponding to human RBPJ. |
| 背景 | RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 68 kDa |
| 参考文献 |
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RBPJ antibody (mAb) tested by ChIP. Chromatin IP was performed using chromatin of an Epstein-Barr virus infected lymphoblastoid cell line ( 2 x 10^6 cell eqivalents per ChIP) and RBPJ antibody or the equivalent amount of IgG negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs for a positive and negative control region. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG. | |
RBPJ antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with RBPJ antibody at a 0.5 µg/ml dilution. |
