| 货号 | 61386 |
| 克隆类别 | 2C5 |
| 同种亚型 | IgG2a |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rat |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunohistochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µl per ChIP ChIP-Seq: 5 - 10 µl each ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. Published Applications: IHC WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This antibody was raised against a full-length protein corresponding to mouse SRF. |
| 背景 | SRF (serum response factor) is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5 of the site of transcription initiation of some genes (such as FOS). This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is amember of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 67 kDa |
| 参考文献 |
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SRF antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse heart and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify SRF binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. |
