| 货号 | 61174 |
| 克隆类别 | 1Tb-1A9 |
| 同种亚型 | IgG2b |
| 反应种属 | Human, Mouse |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 0.5 µg/ml dilution Published Applications: ChIP ICC/IF WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This TRIM28 antibody was raised against a full-length protein corresponding to mouse TRIM28. |
| 背景 | Tripartite Motif-containing Protein 28 (TRIM28), also known as KAP1 (KRAB Associated Protein 1, and TIF1β, Transcriptional Intermediary factor 1β, is an important co-repressor protein that plays a critical role in proliferation and differentiation of both normal and tumor cells. It functions as a co-repressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs) and mediates gene silencing by recruiting CHD3, a subunit of the Nucleosome Remodeling and Deacetylation (NuRD) complex. Part of its mechanism involves coordinating the increase in H3K9 methylation at promoters of silenced genes, potentially through direct recruitment of SETDB1, an H3K9 methyltransferase involved in the formation of heterochromatin. TRIM28 is also involved in maintaining pluripotency and is required for terminal differentiation of embryonic stem cells. TRIM28 protein levels are increased in liver, gastric, lung, breast, and prostate cancer, and gastric cancer patients with high levels of TRIM28 show a significantly lower survival rate. TRIM28 contains two zinc fingers and a PHD finger. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 90 kDa |
| 参考文献 |
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TRIM28 antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with TRIM28 antibody at a 0.5 µg/ml dilution. |
