| 货号 | 61170 |
| 克隆类别 | 195C |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunohistochemistry/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 0.5 µg/ml dilution IHC (paraffin): User optimization required WB: 0.5 - 2 µg/ml dilution Published Applications: IHC WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This WRN antibody was raised against a recombinant protein corresponding to amino acids 1072 - 1432 of human WRN. |
| 背景 | WRN protein is a RECQ helicase that contains 3′ to 5′ helicase activity and DNA-dependent ATPase activity. WRN takes part in many processes, including DNA replication, recombination, repair, and telomere maintenance. Mutations in the gene encoding WRN cause an autosomal recessive segmental progeria called Werner syndrome (WS). WS patients prematurely develop atrophic skin, thin gray hair, osteoporosis, type II diabetes, cataracts, arteriosclerosis and cancer. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 162 kDa |
| 参考文献 |
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WRN antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with WRN antibody at a 0.5 µg/ml dilution. | |
WRN antibody (mAb) tested by Immunohistochemistry. Paraffin embedded tissue samples: human tonsil, MDA-MB-231 (breast adenocarcinoma cell line), AG11395 WS (fibroblast of a patient with Werner Syndrome immortalized with SV40), HeLa cells, MRC-5 (lung fibroblast cell line), and MCF-7 (breast carcinoma cell line). | |
WRN antibody (mAb) tested by Western blot. Detection of WRN by Western blot. Lane 1: HeLa cell extract. Lane 2: WS cell extract (fibroblast from a patient with Werner Syndrome). |
