| 货号 | 61150 |
| 克隆类别 | NIZ 8G5 |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Rat |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Notch3 antibody was raised against a recombinant protein corresponding to the intracytoplasmic portion of human Notch3. |
| 背景 | Notch3 is a member of the Notch family of receptors, which make up a highly conserved cell signaling system. Notch3 functions as a ligand-activated transcription factor that directly transduces extracellular signals in the cell surface into changes in gene expression in the nucleus. Notch3 is membrane bound, serving as an extracellular receptor for the Delta and Serrate family of ligands. Upon activation, the intracellular domain is cleaved and rapidly translocates into the nucleus where it forms a transcriptional complex with the RBP-J DNA binding protein and activates gene expression via recruitment of co-activators of the MAML family. Notch receptors play a critical role in cell fate specification during development and participate in multiple biological processes. Notch signaling is required for undifferentiated embryonic stem cells (ESCs) to participate in the formation of all three embryonic germ layers. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 90 & 270 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Notch3 mAb tested by Western blot. Detection of Notch3 by Western blot. The analysis was performed using 50 µg HeLa whole-cell extract and Notch3 mAb at a 2 µg/ml dilution. |
