| 货号 | 61084 |
| 克隆类别 | 3E10 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rat |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunoprecipitation/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 20 µg per ChIP ChIP-Seq: 20 µg each Published Applications: ChIP IP ICC/IF WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2. |
| 背景 | RNA pol II(RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 240 kDa |
| 参考文献 |
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RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 10 µg of chromatin from human myeloma LP1 cells and 20 µg RNA pol II CTD phospho Ser2 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 2.3 million HL-60 cells and 10 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify RNA pol II phospho Ser2 binding. Data is compared to ChIP-Seq data using a phospho Ser5 antibody (61085). ChIP-Seq data from three specific genes is shown as an example. The Pol II phospho Ser2 antibody detects polymerase more toward the 3´ end of the genes and the phospho Ser5 antibody detects Pol II more at the 5´ end of the genes. |
