| 货号 | 39938 |
| 克隆类别 | 2A8 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot/Immunoprecipitation/Immunofluorescence/Immunohistochemistry/Immunocytochemistry |
| 使用方法 | Validated Applications: IP: 5 µg per IP Published Applications: ChIP IP WB ICC/IF IHC RIP-ChIP HITS-CLIP See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Ago1/2/3 antibody was raised against a recombinant protein corresponding to amino acids 47-859 of human Ago2. |
| 背景 | TheArgonaute family of proteins (Ago1,Ago2,Ago3andAgo4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Ago3 lacks RNase activity and does not appear to cleave target mRNA molecules. Argonaute family proteins contain a PAZ and a PIWIdomain. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 95 kDa |
| 参考文献 |
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Ago1/2/3 antibody (mAb) (Clone 2A8) tested by immunoprecipitation.The Active Motif miRNA IP Kit was used on samples of HT1080 cells that had been transfected with a miR-122 or a non-targeting miRNA control for 8 hours. Following IP using the Ago1/2/3 antibody or negative control IgG, qRT-PCR was performed on the samples using primers for G6PC3 and SLC1A5, which are known targets of miR-122, and for GAPDH, a common housekeeping gene that is not known to be targeted by miR-122. |
