| 货号 | 39620 |
| 克隆类别 | 1H12 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Mouse |
| 应用 | Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1 µg/ml dilution This CENP-E antibody can be used in Western blot to detect CENP-E if the protein has been first enriched by immunoprecipitation. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This CENP-E antibody was raised against full-length human CENP-E protein. |
| 背景 | Centrosome-associated protein E(CENP-E) is a kinesin-like protein and an integral component of kinetochore fibers that link centromeres to spindle microtubules. CENP-E localizes to kinetochores throughout all phases of mitotic chromosome movement. After the onset of anaphase it is released, and by telophase becomes bound to interzonal microtubules of the mitotic spindle. It is likely that CENP-E serves as a chromosome motor during anaphase. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 320 kDa |
| 参考文献 |
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CENP-E mouse mAb (Clone 1H12) tested by immunofluorescence. Staining of HeLa cells with CENP-E mouse mAb (Clone 1H12) at a 1 µg/ml dilution (red) and DAPI (blue). |
