| 货号 | 39558 |
| 克隆类别 | WC119.2H11 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: WB: 1 - 5 µg/ml dilution Published Applications: ICC/IF See reference for experimental details. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA. |
| 背景 | HIRA(DGCR1,TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1α is involved in the formation of the senescence-associated heterochromatin foci. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 120 kDa |
| 参考文献 |
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HIRA mAb (Clone WC119.2H11) tested by Western blot. 5 µg of HIRA mAb (Clone WC15) was used to immunoprecipitate HIRA from 500 µg of colcemid-treated U2OS nuclear extract (lane 2). 5 µg of control mouse IgG was also used (lane 1). The immunoprecipitated protein was detected by Western blotting using HIRA mAb (Clone WC119.2H11) at a dilution of 5 µg/ ml. |
