| 货号 | 39554 |
| 克隆类别 | DO1 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunoprecipitation |
| 使用方法 | Validated Applications: IP: 5 µg per IP WB: 0.5 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This p53 antibody was raised against full-length recombinant human p53 protein. |
| 背景 | p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, a ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 53 kDa |
| 参考文献 |
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p53 mAb (Clone DO1) tested by Western blot. 5 µg of p53 mAb (Clone DO1) was used to immunoprecipitate p53 from 500 µg of camptothecin-treated U2OS nuclear extract (lane 2). 5 µg of mouse IgG was also used as a control (lane 1). The immunoprecipitated protein was detected by western blotting using the same p53 antibody. | |
p53 mAb (Clone DO1) tested by Western blot. Western Blot: Nuclear extract of U2OS cells (20 μg) probed with p53 mAb (Clone DO1) (1 µg/ml). Lane 1: No treatment. Lane 2: Cells treated with camptothecin. |
