| 货号 | 39534 |
| 克隆类别 | 3F3 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: WB: 1:250 - 1:1,000 dilution ICC/IF: 1:1,000 - 1:5,000 dilution Published Applications: see references |
| 供应商 | Active Motif | |
| 纯化方式 | Culture Supernatant | |
| 免疫原 | This HDAC2 antibody was raised against a KLH-conjugated peptide corresponding to amino acids 473-488 of human HDAC2. | |
| 背景 | HDAC2(Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs)andhistone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 55 kDa |
| 参考文献 |
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HDAC2 mAb (Clone 3F3) tested by Western blot. Detection of HDAC2 by Western blot. The analysis was performed using HeLa nuclear extract and HDAC2 mAb (Clone 3F3) at a 1:5,000 dilution. |
