| 货号 | 39532 |
| 克隆类别 | 10E2 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Mouse, Rat |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot/Immunoprecipitation/Immunofluorescence/Immunohistochemistry/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:500 - 1:1,000 dilution WB: 1:500 - 1:2,000 dilution Published Applications: ChIP IP IHC See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif | |
| 纯化方式 | Culture Supernatant | |
| 免疫原 | This HDAC1 antibody was raised against a KLH-conjugated peptide corresponding to amino acids 467-482 of human and murine HDAC1. | |
| 背景 | HDAC1(Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs)andhistone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 60 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
HDAC1 antibody (mAb) (Clone 10E2) tested by immunofluorescence. Top left: HeLa cells stained with HDAC1 antibody (mAb) (Clone 10E2) (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images. | |
HDAC1 antibody (mAb) (Clone 10E2) tested by Western blot. Detection of HDAC1 by Western blot analysis.Nuclear extract of HeLa cells (30μg) was probed with HDAC1 antibody (mAb) (Clone 10E2) at a 1:500 dilution. |
