| 货号 | 39530 |
| 克隆类别 | 10H11.E.12 |
| 同种亚型 | IgG1k |
| 反应种属 | Human, Mouse |
| 来源宿主 | Mouse |
| 应用 | Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:500 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Ascites |
| 免疫原 | This ATM phospho Ser1981 antibody was raised against a peptide containing phospho-serine 1981 of human ATM. |
| 背景 | ATM–Ataxia Telangiectasia Mutated is a PI3-family protein kinase and a critical cell cycle checkpoint protein. ATM and a related protein, ATR, play crucial roles in the maintenance of genome integrity and the response to DNA damage. Upon activation, ATM phosphorylates itself at serine 1981 and then phosphorylates a number of downstream proteins (e.g. BRCA1, CHK2, H2AX, p53, NBS1, SMC1) resulting in cell cycle arrest and the initiation of DNA damage repair. Loss of function of ATM is causal to a variety of syndromes involving increased incidence of several cancers. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Ascites containing 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 370 kDa |
| 参考文献 |
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ATM phospho Ser1981 antibody (Clone 10H11.E.12) tested by immunofluorescence. HeLa cells stained with ATM phospho Ser1981 antibody (Clone 10H11.E.12) at a 1:500 dilution using using MAX Stain™ Immunofluorescence Tools. Panel A: Normal HeLa cells. Panel B: HeLa cells treated with 3 Gy ionizing radiation and collected 30 minutes post-exposure. Top images: Cells stained with DAPI. Middle images: Same cells stained with ATM phospho Ser1981 mAb (Clone 10H11.E.12). Bottom images: Merge of the two images above. Note the Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of ATM at serine 1981. In contrast, Panel A-middle image shows no detectable phosphorylated ATM. |
