| 货号 | 39528 |
| 克隆类别 | 5-B-1-2 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,500 dilution ICC/IF: 1:1,000 - 1:10,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Ascites |
| 免疫原 | This alpha Tubulin antibody was raised against total protein purified from sea urchin sperm axonemes. |
| 背景 | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of α-tubulinandβ-tubulin.Alpha Tubulin expression is reduced in cells deficient in MeCP2, leading to conditions such as Rett syndrome and autistic-spectrum disorders. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Ascites containing 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 52 kDa |
| 参考文献 |
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alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence. Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain™ Immunofluorescence Tools. | |
alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot. HeLa whole-cell extract (15 µg/lane) probed with alpha Tubulin mAb (1:5,000 dilution). |
