| 货号 | 39384 |
| 克隆类别 | 52A311 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 0.5 µg/ml dilution WB: 0.5 - 2 µg/ml dilution |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Rad21 antibody was raised against recombinant protein corresponding to the N-terminal half of mouse Rad21. |
| 背景 | Rad21Clone 52A311(HR21,MCD1,NXP1,hHR21) is highly similar to the gene product of S. pombeRad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. Rad21 is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of Rad21 with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 100 kDa |
| 参考文献 |
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Rad21 antibody (mAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with Rad21 antibody at a 0.5 µg/ml dilution. | |
Rad21 antibody (mAb) tested by Western blot. HeLa nuclear extract (30 µg per lane) was probed with Rad21 mAb (Clone 52A311) (1 µg/ml). |
